A Random Walk in Science, by Robert L. Weber

By Robert L. Weber

This anthology offers an perception into the wit and mind of the medical brain via a mix of a laugh and severe contributions written through and approximately scientists. The contributions checklist altering attitudes inside of technology and reflect the interactions of technology with society.

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Compared with other used methods, such as phenol/chloroform extraction, TRIzol extraction and denaturation by 7 M urea, the heating method is simple and efficient. That is because: (i) Elevated temperature will cause denaturing of the cell surface proteins and the folded structure of the DNA, and this will lead to the disruption of the interaction between DNA and protein and the release of DNA from the target protein. (ii) At 95 °C, any DNase that is released after cell disruption at the elevated temperature is inactivated and therefore cannot cause DNA digestion.

1021/cn100114k 30. Jimenez E, Sefah K, Lopez-Colon D, Van Simaeys D, Chen HW, Tockman MS, Tan W (2012) Generation of lung adenocarcinoma DNA aptamers for cancer studies. PLoS ONE 7 (10):e46222. 0046222 31. Bing T, Yang XJ, Mei HC, Cao ZH, Shangguan DH (2010) Conservative secondary structure motif of streptavidin-binding aptamers generated by different laboratories. Bioorg Med Chem 18(5):1798–1805. 054 32. Shangguan D, Tang ZW, Mallikaratchy P, Xiao ZY, Tan WH (2007) Optimization and modifications of aptamers selected from live cancer cell lines.

For those who is willing to add functional groups onto natural DNA, the inevasible enzymatic polymerization reactions for amplification involved in SELEX imposes the restriction that the modified NTP has to be perfectly compatible with the usually over 200 rounds of amplification 3 Unnatural Nucleic Acids for Aptamer Selection 39 during SELEX process. By mentioning compatible here, it means these functional groups added should not interfere with the recognition and binding sites of polymerase and should also match with the complementary nucleotides.

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