By Michael W. W. Adams, Robert M. Kelly
content material: Biocatalysis close to and above 100°C : an outline / Michael W.W. Adams and Robert M. Kelly --
Metabolic enzymes from sulfur-dependent, super thermophilic organisms / Michael W.W. Adams ... [et al.] --Characterization of enzymes from high-temperature micro organism / Robert M. Kelly ... [et al.] --
Thermally solid urease from thermophilic micro organism / Kenneth Runnion, Joan Combie, and Michael Williamson --
breathing electron-transport parts in hyperthermophilic micro organism / R.J. Maier, L. Black, T. Pihl, and B. Schulman --
Key enzymes within the basic nitrogen metabolism of a hyperthermophile / Frank T. Robb .. [et al.] --
Biocatalysis in natural media / Don A. Cowan and Adrian R. Plant --
strain dependence of enzyme catalysis / Peter C. Michels and Douglass S. Clark --
Thermodynamic thoughts for protein layout : elevated temperature balance / Martin Straume, Kenneth P. Murphy, and Ernesto Freire --
balance of hot temperature enzymes : improvement and trying out of a brand new predictive version / Bruce E. Dale and John P. McBennett --
Computational techniques to modeling and studying thermostability in proteins / John E. Wampler ... [et al.] --
DNA-binding proteins and genome topology in thermophilic prokaryotes / D.R. Musgrave ... [et al.] --
purposes of thermostable DNA polymerases in molecular biology / E.J. Mather.
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Extra resources for Biocatalysis at Extreme Temperatures. Enzyme Systems Near and Above 100 °C
However, if enzyme expression is maximal under limiting nutrient conditions, the overall yield for the enzyme may be extremely low. This was found to be the case in the production of proteolytic activities from P. ftariosus. As is the case with amylolytic enzymes from this particular bacterium (Brown and Kelly, submitted), the optimization of enzyme expression through nutritional experiments may be more important than with mesophilic systems. ch003 Choice of Enzyme Thus far, the choice of enzyme to study from high temperature bacteria has been based on existing expertise with comparable enzymes from less thermophilic origins or driven by biotechnological potential.
3. KELLY ET AL. , in press). Several amylolytic activities have also been found in P. furiosus (17) including a-glucosidase, pullulanase and amylase. These activities have been found in many high temperature heterotrophs. Such hydrolases are, of course, potentially important as commercial enzymes. ch003 Purification Protocols Assuming that sufficient biomass can be generated containing an enzyme or enzymes of interest, purification protocols must be developed. Thus far, existing methodologies can be adapted and have been used in most cases reported to date.
Fortunately, further efforts with the co-purified protein, PI (to this point, its function has not been identified), resulted in meaningful calorimetry experiments as shown in Figure 5. This protein is a homo te tramer with a molecular weight of approximately 140 kDa. , in preparation). In hindsight, the choice of the α-glucosidase as a model hyperthermophilic protein was both good and bad. Because of its physiological significance, ease of assay at elevated temperatures, and numerous counterparts, there are advantages to its choice.