Biomedical Electron Microscopy. Illustrated Methods and by Arvid B. Maunsbach, Björn A. Afzelius

By Arvid B. Maunsbach, Björn A. Afzelius

  • ''The booklet incorporates a wealth of data at the interpretation of electron micrographs - in a level of element that typically is understood purely at the paintings flooring itself... This ebook has safeguarded very important features of electron microscopy for destiny morphologists.''
    --TRENDS IN phone BIOLOGY


    ''This is a wonderful e-book that are supposed to be within the arms of any new or skilled practitioner of electron microscopy. The writing is obvious and direct and the micrographs set the top average of procedure, caliber, and value. it is going to set a brand new general of excellence in reinforcing the significance of the cautious and applicable use of electron microscopy in smooth mobile biology. No collage, collage, or existence technological know-how library might be with no it.''
    --DOODY'S PUBLISHING studies


    ''It needs to be the main entire choice of artifacts to be published!...The energy of the e-book is within the transparent and succinct presentation of concepts; the big variety of micrographs illustrating the implications; the various examples of artifacts and different defects; the top of the range details given approximately specimen practise for every instance; and the references on the finish of every chapter.''
    --MICRON


    ''This beautifully illustrated assortment would definitely obtain very heavy use...''
    —ULTRAMICROSCOPY (1999)


    ''The actual price of this ebook is that it offers a identified and high quality reference commonplace opposed to which one's personal paintings could be judged and seriously evaluated. it truly is of profit to all those that paintings within the box of organic transmission electron microscopy, in addition to the scientific box indicated within the title...''
    —PROCEEDINGS OF THE ROYAL MICROSCOPICAL SOCIETY (1999)

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Sample text

F. , and Klomparens, K. L. ) (1988). " Plenum Press, New York/London. , JCrgensen, P. , and Maunsbach, A. B. (1977). Ultrastructure of the sodium pump: Comparison of thin sectioning, negative staining, and freeze-fracture of purified, membrane-bound (Na +, K+)-ATPase. J. Cell BioL 75, 619-634. , and Afzelius, B. (1994). Three-dimensional reconstructions of accessory tubules observed in the sperm axonemes of two insect species. J. Struct. Biol. 113, 225-237. Dvorak, A. M. (1987). Procedural guide to specimen handling for the ultrastructural pathology service laboratory.

2. 2, dehydrated in acetone, and embedded in Vestopal. Thin sections were stained with lead citrate only. • 115,000. 9B Same kind of specimen as in Fig. 1 M phosphate buffer and then postfixed in 1% uranyl acetate in distilled water, epoxy embedded, sectioned, and section stained with uranyl acetate and lead citrate, x 85,000. Block staining with uranyl acetate reveals cytological details that may be difficult to visualize by conventional section staining with uranyl and lead salts. In addition, the image often appears more crisp.

Invest. 25, 1-14. Maunsbach, A. B. (1966). Absorption of 1125-labeled homologous albumin by rat kidney proximal tubule cells: A study of microperfused single proximal tubules by electron microscopic autoradiography and histochemisty. J. Ultrastr. Res. 15, 197-241. Maunsbach, A. B. (1976). Cellular mechanisms of tubular protein transport. ), Vol. II, pp. 145-167. University Park Press, Baltimore. Maunsbach, A. , Ning, G. , and Nielsen, S. (1997). Aquaporin-1 water channel expression in human kidney.

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